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Recombinant Mouse sTfR/CD71 Protein (RP01497)

Recombinant Mouse sTfR/CD71 Protein was resolved with SDS PAGE under reducing (R) conditions

Immobilized Mouse sTfR/CD71 (Catalog: RP01497) at 5 μg/mL (100 μL/well) can bind Mouse Transferrin/TF (Catalog: RP01603) with a linear range of 0.002-1.8 ng/mL.

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货号: RP01497
促销价:   ¥2500
货    期:现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
Calculated MW
76.74 kDa
Observed MW
80-90 kDa
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
<0.1EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Mouse sTfR/CD71 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys89-Phe763) of mouse Transferrin R/CD71 (Accession #NP_035768.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA.Immobilized Mouse sTfR/CD71 (Catalog: RP01497) at 5 μg/mL (100 μL/well) can bind Mouse Transferrin/TF (Catalog: RP01603) with a linear range of 0.002-1.8 ng/mL.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

This protein is a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.

基因ID
Swiss Prot
别名
CD71;IMD46;p90;T9;TFR;TFR1;TR;TRFR;TFRC
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