Recombinant Mouse PVR/CD155 Protein (RP01557)

说明书联系我们收藏

Recombinant Mouse PVR/CD155 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-100kDa.

Immobilized Mouse CD155 at 2 μg/mL (100 μL/well) can bind Mouse TIGIT with a linear range of 0.3-4.27 ng/mL.

All(2)|

货号: RP01557

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

62.54 kDa

Observed MW

80-100 kDa

标签

C-hFc&Avi

纯度

> 95% by SDS-PAGE.

内毒素

<0.1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Mouse PVR/CD155 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp29-Leu348) of mouse PVR/CD155 (Accession #NP_081790.1) fused with a Fc, Avi tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Mouse CD155 at 2 μg/mL (100 μL/well) can bind Mouse TIGIT with a linear range of 0.3-4.27 ng/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

The recombinant human PVR consists of 323 amino acids with a molecular weight of 35.1 kDa.CD155 is also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) It is a type I transmembrane single-span glycoprotein belonging to the nectins and nectin-like (Necl) subfamily. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system.Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.

基因ID

52118

Swiss Prot

Q8K094

别名

CD155;HVED;Necl-5;NECL5;PVS;TAGE4;PVR;CD155;HVED;Necl-5;NECL5;PVS;TAGE4;PVR