Recombinant Human CCL5/RANTES Protein (RP01563LQ)

Recombinant Human CCL5/RANTES Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 10-15 kDa

50 ng/mL of Recombinant Human CCL5/RANTES enhances STAT3,P65 autophosphorylation and upregulates the expression of MDA5 in SW620 cells.

All(2)|

货号: RP01563LQ

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

8.69 kDa

Observed MW

12-15 kDa

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

<1EU/μg

制剂

Supplied as a 0.22 μm filtered solution in 50 mM Tris,500 mM NaCl,5% glycerol,pH7.5.

描述

Recombinant Human CCL5/RANTES Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser24-Ser91) of human CCL5/RANTES (Accession #NP_002976.2) fused with a 6×His tag at the C-terminus

储存

Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

50 ng/mL of Recombinant Human CCL5/RANTES enhances STAT3,P65 autophosphorylation and upregulates the expression of MDA5 in SW620 cells.

复溶

Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

基因ID

6352

Swiss Prot

P13501

别名

CCL5;D17S136E;RANTES;SCYA5;SIS-delta;SISd;TCP228;eoCP

研究领域