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Recombinant Human CCL5/RANTES Protein (RP01563LQ)

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Recombinant Human CCL5/RANTES Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 9 kDa

50 ng/mL of Recombinant Human CCL5/RANTES enhances STAT3,P65 autophosphorylation and upregulates the expression of MDA5 in SW620 cells.

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货号: RP01563LQ
促销价：   ￥950
货    期：现货产品
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详细信息

种属
Human
表达宿主
E. coli
描述
Recombinant Human CCL5/RANTES Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser24-Ser91) of human CCL5/RANTES (Accession #NP_002976.2) fused with a 6×His tag at the C-terminus
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
<1EU/μg
生物活性
50 ng/mL of Recombinant Human CCL5/RANTES enhances STAT3,P65 autophosphorylation and upregulates the expression of MDA5 in SW620 cells.
制剂
Supplied as a 0.22 μm filtered solution in 50 mM Tris,500 mM NaCl,5% glycerol,pH7.5.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

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背景信息

Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

基因ID
6352
Swiss Prot
P13501
别名
CCL5;D17S136E;RANTES;SCYA5;SIS-delta;SISd;TCP228;eoCP
研究领域

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