产品和服务 / 产品类型 / 重组蛋白 / 生长因子

活性验证

Recombinant Human Hepatocyte growth factor/HGF Protein (RP01602)

评论 (2) 说明书 收藏

Recombinant Human Hepatocyte growth factor/HGF Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Recombinant Human Hepatocyte growth factor/HGF Protein is greater than 95% as determined by SEC-HPLC.

Recombinant Human HGF induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg.

Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 2.66-10.64 ng/mL,corresponding to a specific activity of 9.40×104~3.76×105 units/mg.

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

All(5)|
货号: RP01602
促销价：   ￥780
货    期：现货产品
抗体定制
服务咨询 |
扫码下单
享受积分

详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
80.51 kDa
Observed MW
100-110 kDa
标签
C-His
纯度
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human Hepatocyte growth factor/HGF (Accession #NP_000592.3) fused with His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12×105-4.48×105 units/mg.2.Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 2.66-10.64 ng/mL,corresponding to a specific activity of 9.40×104~3.76×105 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

客户数据及评论 (2)

折叠内容
中国医学科学院血液病医院 (血液学研究所)Nov 122024
评分
Experiment Type
Cell-based assay
Sample
Mesendoderm cells
Description
Combined treatment of recombinant HGF (20 ng/mL, Cat. RP01602) and KGF (20 ng/mL, Cat. RP02882LQ) could induce double negative mesendoderm cells differentiation into hepatocytes with AFP/SOX9 double positive.
中国中医科学院医学实验中心Oct 232024
评分
Experiment Type
Cell-based assay
Sample
iPSC
Description
The induced pluripotent stem cells (iPSCs) were incorporated into the prepared culture medium in accordance with the established induction protocols at various stages of culture. The medium contained Activin A (100 ng/mL, Cat. RP02516), bFGF (10 ng/mL, Cat. RP01042), HGF (20 ng/mL, Cat. RP01602), and Oncostatin M (10 ng/mL, Cat. RP00054), all sourced from ABclonal, to facilitate the successful growth of liver organoids.

背景信息

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis,and tissue regeneration.

基因ID
3082
Swiss Prot
P14210
别名
HGF;DFNB39;F-TCF;HGFB;HPTA;SF
研究领域