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Recombinant Human Wnt-3a Surrogate Protein (RP01618SLQ)

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Human gastric cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), FGF10(Cat. RP01140), RSPO1(Cat. RP00071), Wnt-3a Surrogate(Cat. RP01618SLQ), A83-01(Cat. RPM0008), Y-27632 dihydrochloride(Cat.RPM0007), Gastrin Ⅰ(Cat. RPM0011), AB-27 Supplement(Cat. RPM0047LQ), N-2 Supplement(Cat. RPM0046LQ).

Recombinant Human Wnt-3a Surrogate Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.

Mouse lung organoids were cultured in Matrigel (Cat. RPM0003) with medium supplemented with EGF (Cat. RP03287), Noggin (Cat. RP01237), RSPO1 (Cat. RP00071), Wnt-3a (Cat. RP01618SLQ), and FGF-10 (Cat. RP01140). After 8 days of culture, the expression of lineage markers, including E-Cadherin (Cat. A20798), AQP5 (Cat. A23292), and CD49F (Cat. A26707), was assessed by ultra-plex immunofluorescence staining.

Human stomach organoids organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 7.38-29.54 ng/mL ,corresponding to a specific activity of 3.39×104~1.35×105 units/mg.

Mouse large intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ)

Recombinant Human Wnt-3a Surrogate Protein is greater than 95% as determined by SEC-HPLC.

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

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货号: RP01618SLQ
价 格：  950.00
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详细信息

靶点
Wnt Surrogate Protein
细胞系
67.52 kDa
突变描述
Supplied as 0.22 μm filtered solution in PBS buffer with Trehalose, Glycerol, and Tween80, pH 7.4.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
66-75 kDa
物种
HEK293 Cells
保存条件
Store at -80℃
运输条件
HEK293 Cells

客户数据及评论 (2)

折叠内容
中国科学院动物研究所Nov 282025
评分
Experiment Type
Cell-based assay
Sample
hPSC
Description
The differentiation medium containing Wnt-3a (500 ng/mL) and FGF-4 (500 ng/mL) was used to induce the differentiation of definitive endoderm (DE) cells into mid-hindgut cells. After 4 days of induction, the expression of the mid-hindgut marker CDX2 was assessed by immunofluorescence.
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厦门百脑慧医学研究有限公司Jul 282025
评分
Experiment Type
Cell-based Assay
Sample
prostate cancer cells
Description
The picture show culture of human prostate cancer cells using Wnt-3a (RP01618SLQ), Noggin (RP01237), FGF-10 (RP01140), bFGF (RP01042), RSPO1 (RP00071), and EGF (RP01030).
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背景信息

Wnt3a Surrogate protein is a Wnt recombination protein that plays an important role in embryonic development, adult tissue homeostasis and injury repair. Wnt3a Surrogate protein, consisting of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth. Wnt3a or the small molecule glycogen synthase kinase 3 (GSK3) inhibitor CHIR are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist.

别名
WNT3A;Protein Wnt-3a;Wnt3a Surrogate