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活性验证

Recombinant Mouse Thrombopoietin/THPO Protein (RP01665S)

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Measured in a cell proliferation assay using M07e human megakaryocytic leukemic cells. The ED50 for this effect is 0.028‑0.110 ng/mL, corresponding to a specific activity of 9.07×106~3.62×107units/mg.

Recombinant Mouse Thrombopoietin/THPO Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

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货号: RP01665S
价 格：  780.00
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详细信息

靶点
Thpo
细胞系
20.08 kDa
突变描述
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
15-25 kDa
产品组成
Ser22-Ser184
物种
HEK293 cells
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

背景信息

Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.

基因ID
21832
Swiss Prot
P40226
别名
Thpo;Thrombopoietin; MGDF; THPO;TPO