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Recombinant Mouse IL-12/IL-12A&IL-12B Protein (RP01691)

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Recombinant Mouse IL-12A&IL-12B Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) kDa.

Immobilized Mouse IL-12A&IL-12B (Catalog: RP01691) at 5 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.

Recombinant Mouse IL-12A&IL-12B stimulates cell proliferation of the IL2-activated mouse splenocytes. The ED50 for this effect is 0.01- 0.03 ng/mL, corresponding to a specific activity of 3.3×107~ 1× 10 8 units/mg.

Recombinant Mouse IL-12/IL-12A&IL-12B induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 7.3-29.2 ng/mL, corresponding to a specific activity of 3.4×104~1.4×105 units/mg.

Active Recombinant Mouse IL-12(Cat. RP01691)induces the mouse T cells differentiate into Th1 cells. Flow cytometry was used to detect the expression of IFN-γ. Result showed that Th1 cells were induced successfully.(Customer Feedback Data).

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货号: RP01691
促销价：   ￥1100
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Active Recombinant Mouse IL-12/IL-12A&IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg23-Ala215＆Met23-Ser335) of Mouse IL-12A&IL-12B (Accession #P43431＆P43432) fused with and a 6×His tag at the C-terminus(IL-12).
标签
C-His(IL-12a)&No tag(IL-12b)
纯度
> 95% by SDS-PAGE.
内毒素
<0.1EU/μg
生物活性
1.Measured by its binding ability in a functional ELISA.Immobilized Mouse IL-12A&IL-12B (Catalog: RP01691) at 5 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.
2.Measured in a cell proliferation assay using IL2-activated mouse splenocytes. The ED50 for this effect is 0.01- 0.03 ng/mL, corresponding to a specific activity of 3.3×107~ 1× 10 8 units/mg.
3.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 7.3-29.2 ng/mL, corresponding to a specific activity of 3.4×104~1.4×105 units/mg.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

基因ID
16159&16160
Swiss Prot
P43431＆P43432
别名
p35; Ll12a; Il-12a; IL-12p35&p40; Il-12b; Il12p40; Il-12p40;IL-12&Il12b;IL-12 p70（IL12A&IL12B）
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