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Recombinant Human IL-15 Protein (RP01751)

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Recombinant Human IL-15 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55-60 kDa.

Recombinant Human IL-15 stimulates cell proliferation of the HT-2 mouse T cells. The ED50 for this effect is 27-108 ng/mL, corresponding to a specific activity of 9.26 ×103-3.70×104 units/mg.

All(2)|
货号: RP01751
促销价：   ￥780
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human IL-15 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn49-Ser162) of Human IL-15 (Accession #NP_000576.1) fused with and a hFc tag at the C-terminus.
标签
C-hFc
纯度
> 95% by SDS-PAGE.
内毒素
< 1EU/μg
生物活性
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 27-108 ng/mL, corresponding to a specific activity of 9.26 ×103-3.70×104 units/mg.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Mouse interleukin-15 receptor subunit alpha, also known as Il15ra, is a high-affinity receptor for interleukin-15.Il15ra associates as a heterotrimer with the IL-2 receptor beta and gamma subunits (Common gamma chain, orgamma c) to initiate signal transduction. It can signal both in cis and trans where IL15R from one subset of cellspresents IL15 to neighboring IL2RG-expressing cells. Il15ra is expressed in special cells including a wide varietyof Tand B cells and non-lymphoid cells. Human Il15ra shares 45% amino acid sequence homology with themouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified, resulting fromalternative splicing events involving different exons.

基因ID
3600
Swiss Prot
P40933-1
别名
IL-15；IL15
研究领域
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