Recombinant Human Interferon omega-1/IFNW1 Protein (RP01752)

Recombinant Human Interferon omega-1/IFNW1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 22 kDa.

Measured in a cell cytotoxicity assay using TF-1 cells. The ED₅₀ for this effect is 0.13-0.54 ng/mL, corresponding to a specific activity of 1.85 × 10⁶~7.69× 10⁶ units/mg.

All(2)|

货号: RP01752

详细信息

种属

Human

表达宿主

HEK293 cells

描述

Recombinant Human Interferon omega-1/IFNW1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly23-Ser195) of human Interferon omega-1/IFNW1 (Accession #NP_002168.1) fused with a 6×His tag at the C-terminus.

标签

C-6His

纯度

> 97% by SDS-PAGE.

内毒素

< 0.1EU/μg

生物活性

Measured in a cell cytotoxicity assay using TF-1 cells. The ED₅₀ for this effect is 0.13-0.54 ng/mL.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

IFNs are a large family of proteins having antiviral, antiproliferative, and immunomodulatory effects, and are divided into two major classes, type I and type II, based on differences in receptor binding and nucleotide sequence. Type I IFNs consist of IFN α, β, τ, and ω and bind to the type I IFN receptor, whereas IFN-γ is the only type II IFN and is specific for the type II IFN receptor. Human IFN-ω, was identified by three independent groups in 1985 and is structurally related to IFN-α and -β. Both human IFN-ω and IFN-α are produced by virally induced leukocytes and have similar antiviral activities on human cell lines, and a sizeable proportion (at least 1%) of the total antiviral activity of leukocyte IFN is contributed by IFN-ωl. Also, it was reported that IFN-ω could inhibit the growth of human tumors in vivo.

基因ID

3467

Swiss Prot

P05000

别名

Interferon omega-1；IFNW1

研究领域