Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein (RP01782S)

Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions ，showing single bands at 15-20 kDa and 25-30 kDa, respectively.

Measured by its ability to inhibit proliferation of MPC-11 cells. The ED₅₀ for this effect is 3.215-12.86 ng/mL, corresponding to a specific activity of 7.78×10⁴~3.11×10⁵ units/mg.

All(2)|

货号: RP01782S

详细信息

种属

Human

表达宿主

CHO Cells

Calculated MW

12.98 kDa

Observed MW

15-20 kDa

标签

No-Tag

内毒素

< 0.01 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein is produced by CHO cells expression system. The target protein is expressed with sequence (Gly311-Ser426) of Human/Mouse/Rat mature Activin A/INHBA (Accession #NP_002183.1) fused with a His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to inhibit proliferation of MPC-11 cells. The ED₅₀ for this effect is 3.215-12.86 ng/mL, corresponding to a specific activity of 7.78×10⁴~3.11×10⁵ units/mg.

复溶

Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.

基因ID

3624

Swiss Prot

P08476

别名

EDF; FRP; INHBA; Activin A

研究领域