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Recombinant Mouse TNFSF11/RANKL/CD254 Protein (RP01785)

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Recombinant Mouse TNFSF11/RANKL/CD254 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa.

Measured by its ability to induce osteoclast differentiation of mouse bone marrow cells. A combination of recombinant mouse M-CSF (Cat. RP01216, 50 ng/mL) and RANKL (50 ng/mL) can effectively induce the differentiation of mouse bone marrow cells to osteoclasts.

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 11.19-44.76 ng/mL, corresponding to a specific activity of 2.23×104~8.94×105 units/mg.

All(3)|
货号: RP01785
促销价：   ￥1200
货    期：现货产品
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详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse TNFSF11/RANKL/CD254 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg72-Asp316) of mouse TNFSF11 (Accession #NP_035743.2) fused with a 6×His tag at the N-terminus.
标签
N-6His
内毒素
< 0.01 EU/μg
生物活性
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 11.19-44.76 ng/mL, corresponding to a specific activity of 2.23×104~8.94×105 units/mg.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor(TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such asthymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involvedin a number of fundamental biological processes such as acting as regulator of interactions between T-cells anddendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption inhumoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cellproliferation.

基因ID
21943
Swiss Prot
O35235
别名
ODF; OPGL; RANKL; Ly109l; Trance；TNFSF11；CD254
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