Recombinant Human Neurogranin/NRGN Protein (RP01814)

说明书联系我们收藏

Recombinant Human NRGN/Neurogranin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-45 kDa.

All(1)|

货号: RP01814

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

33.45 kDa

Observed MW

45 kDa

标签

C-hFC

纯度

> 97% by SDS-PAGE.

内毒素

< 0.01EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris 150mM Nacl, pH 7.5.

描述

Recombinant Human Neurogranin/NRGN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp2-Asp78) of human NRGN/Neurogranin (Accession #) fused with hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

Neurogranin is as a small protein encoded by the NRGN gene. it is a calmodulin-binding protein expressed primarily in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability. It is a potential biomarker of neurological and mental diseases.

基因ID

4900

Swiss Prot

Q92686

别名

RC3; hng;NRGN;Neurogranin

研究领域