产品和服务 / 产品类型 / 重组蛋白 / 细胞因子 & 细胞因子受体

Recombinant Human Angiopoietin-1/ANG-1/ANGPT1(277-498) Protein (RP01828)

Recombinant Human Angiopoietin-1/ANG-1/ANGPT1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-45 kDa.

All(1)|
货号: RP01828

促销价:   ¥780
货    期:现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
26.58 kDa
Observed MW
30-35 kDa
标签
C-His
纯度
> 92% by SDS-PAGE.
内毒素
< 0.1 EU/μg
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human Angiopoietin-1/ANG-1/ANGPT1(277-498) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg277-Phe498) of human Angiopoietin-1/ANG-1/ANGPT1 (Accession #NP_001137.2) fused with a 6×His tag at the
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

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背景信息

The angiopoietin (ANGPT)-TIE2/TEK signaling pathway is essential for blood and lymphatic vascular homeostasis. ANGPT1 is a potent TIE2 activator, whereas ANGPT2 functions as a context-dependent agonist/antagonist. In disease, ANGPT2-mediated inhibition of TIE2 in blood vessels is linked to vascular leak, inflammation, and metastasis.

基因ID
Swiss Prot
别名
AGP1; AGPT; ANG1; HAE5; AGPT-1;Angiopoietin-1;ANG-1;ANGPT1
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