Recombinant Mouse TGF-alpha Protein (RP01846)

Recombinant Mouse TGF-alpha/TGFA Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 32-35,40 kDa.

Recombinant Mouse TGF-alpha/TGFA stimulates cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.43-1.72 ng/mL, corresponding to a specific activity of 15.81×10⁵~2.33×10⁶ units/mg.

All(2)|

货号: RP01846

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

32.08 kDa

Observed MW

35-45 kDa

标签

C-hFc

内毒素

<0.1EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Mouse TGF-alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala38-Ala88) of Mouse TGF-alpha/TGFA (Accession #NP_112476.1) fused with a hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.43-1.72 ng/mL, corresponding to a specific activity of 15.81×10⁵~2.33×10⁶ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Transforming growth factor alpha (TGF-α) is a protein that in humans is encoded by the TGFA gene. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes, and macrophages.TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

基因ID

21802

Swiss Prot

P48030

别名

Protransforming growth factor alpha; Cleaved into: Transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1，Tgfa