Recombinant Mouse Autotaxin/E-NPP2 Protein (RP01852)

Recombinant Mouse Autotaxin/E-NPP2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-140 kDa.

All(1)|

货号: RP01852

详细信息

种属

Mouse

表达宿主

HEK293 cells

Calculated MW

94.40 kDa

Observed MW

100-130 kDa

标签

C-His

内毒素

<0.1EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150mM NaCl，pH7.4

描述

Recombinant Mouse Autotaxin/E-NPP2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser49-Ile862) of Mouse Autotaxin/E-NPP2 (Accession #NP_056559.2) fused with a His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain . Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine . The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site . Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2 . ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis ( and to be up-regulated in several types of carcinomas including breast and lung .

基因ID

18606

Swiss Prot

Q9R1E6-1

别名

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; 3.1.4.39; Autotaxin; Extracellular lysophospholipase D; LysoPLD，Enpp2; Npps2; Pdnp2