Recombinant Rat Erythropoietin/EPO Protein (RP01858)

Recombinant Rat Erythropoietin/EPO Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa.

Recombinant Rat Erythropoietin/EPO stimulates cell proliferation assay using TF-1 Human erythroleukemic cells. The ED₅₀ for this effect is 0.7-2.8 ng/mL, corresponding to a specific activity of 3.57×10⁵~1.43×10⁶ units/mg.

All(2)|

货号: RP01858

详细信息

种属

Rat

表达宿主

HEK293 cells

Calculated MW

19.36 kDa

Observed MW

30-40 kDa

标签

C-His

内毒素

<0.1EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Rat Erythropoietin/EPO Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg192) of Rat Erythropoietin/EPO (Accession #NP_058697.1) fused with a His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED₅₀ for this effect is 0.7-2.8 ng/mL, corresponding to a specific activity of 3.57×10⁵~1.43×10⁶ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Erythropoietin (EPO), originally identified for its critical hormonal role in promoting erythrocyte survival and differentiation, is a member of the large and diverse cytokine superfamily. EPO and EPOR function as the primary mediators of a general protective response to tissue hypoxia, which acts to maintain adequate tissue oxygenation through adjustments of circulating red cell mass by using a hormonal feedback-control system involving the kidney and the bone marrow. EPO and EPORs are also expressed by other tissues and organs, including the brain and heart. EPO has also been shown to stimulate mitosis and signaling in astrocytes, endothelial cells, cardiomyoblasts, and cardiomyocytes maintained in vitro

基因ID

24335

Swiss Prot

P29676

别名

Erythropoietin，Epo