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活性验证

Recombinant Human IFN-alpha 21 Protein (RP01900)

说明书 收藏

Recombinant Human IFN-alpha 21/IFNA21 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-25 KD.

Recombinant Human IFN-alpha 21/IFNA21 Protein cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.50‑1.98 ng/mL, corresponding to a specific activity of 5.05×105~2.00×106 units/mg.

All(2)|
货号: RP01900

促销价：   ￥780
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
20.15 kDa
Observed MW
20-25 kDa
标签
C-His
内毒素
<0.01EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human IFN-alpha 21 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Glu189) of Human IFN-alpha 21/IFNA21 (Accession #NP_002166.2) fused with a His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 0.50‑1.98 ng/mL, corresponding to a specific activity of 5.05×105~2.00×106 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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背景信息

Interferons (IFN) are a family of cytokines with potent antiviral, anti-proliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors. Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology . Structurally, type I IFNs belong to the class of five helicalbundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds . There is not a mouse homolog for IFNA21, but mature human IFNA21 is identical to chimpanzee IFNA21. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) . Individual IFNA subtypes are known to display unique efficacies to viral protection, with IFNA21 displaying intermediate activity inducing interferon stimulating genes. Further, human IFNA21 has shown weak anti-viral activity against viruses such as metapneumovirus .

基因ID
3452
Swiss Prot
P01568
别名
IFNA21; IFN-alpha 21; IFNalpha F; IFN-alpha F; IFN-alpha-21; IFN-alphaI; interferon alpha-21; Interferon alpha-F; interferon; alpha 21; LeIF F; leukocyte interferon protein; MGC126687; MGC126689
研究领域
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