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活性验证

Recombinant Human IFN-alpha G/IFNA5 Protein (RP01915)

说明书 收藏

Recombinant Human IFN-alpha G/IFNA5 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25 KD.

Recombinant Human IFN-alpha G/IFNA5 was measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 3.27‑13.06 ng/mL, corresponding to a specific activity of 7.66×104~3.06×105 units/mg.

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货号: RP01915

促销价：   ￥620
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
20.53 kDa
Observed MW
20-25 kDa
标签
C-His
内毒素
<0.1EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
描述
Recombinant Human interferon-alpha/IFNA5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu22-Glu189) of Human interferon-alpha/IFNA5 (Accession #NP_002160.1) fused with a His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 3.27‑13.06 ng/mL, corresponding to a specific activity of 7.66×104~3.06×105 units/mg.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

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背景信息

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

基因ID
3442
Swiss Prot
P01569
别名
Ifa5; IFNA5; IFNalpha G; IFN-alpha G; IFN-alpha-5; IFN-alphaG; INFA5; interferon alpha-5; Interferon alpha-61; Interferon alpha-G; interferon; alpha 5; LeIF G
研究领域
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