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Recombinant Cynomolgus PD-1/PDCD1/CD279 Protein (RP02042)

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Cynomolgus PD-1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

The purity of Cynomolgus PD-1 is greater than 95% as determined by SEC-HPLC.

Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L1, His Tag with an affinity constant of 1.28 μM as determined in SPR assay (Biacore T200).

Immobilized Cynomolgus PD-1, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.

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货号: RP02042
促销价：   ￥2700
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详细信息

种属
Cynomolgus
表达宿主
HEK293 cells
描述
Recombinant Human DLL3 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Pro21-Gln167) of cynomolgus PD-1 (Accession #B0LAJ3) fused with an Fc tag at the C-terminus.
标签
C-hFc
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Swiss Prot
B0LAJ3
别名
CD279;hPD-1;hPD-l;hSLE1;PD-1;PD1;SLEB2
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