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Recombinant Cynomolgus CD3 epsilon/CD3E Protein (RP02214)

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Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

Immobilized Cynomolgus CD3E, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 32.7ng/ml determined by ELISA.

All(3)|
货号: RP02214
促销价：   ￥2850
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详细信息

种属
Cynomolgus
表达宿主
HEK293 cells
描述
Recombinant Cynomolgus CD3 epsilon/CD3E Protein is produced by mammalian expression system. The target protein is expressed with sequence (Gln22-Asp117) of cynomolgus Cynomolgus CD3 Epsilon (Accession #) fused with a His tag at the C-terminus.
标签
C-His
纯度
> 95% as determined by Bis-Tris PAGE.> 95% as determined by HPLC
内毒素
< 1EU per μg by the LAL method.
生物活性
Immobilized Cyno CD3 epsilon at 1 μg/mL(100 μL/Well).Dose response curve for Anti-CD3 Ab. with the EC50 of 0.2 μg/mL determined by ELISA.
制剂
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8+ naive T cells) and also T helper cells (CD4+ naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

基因ID
102133065
Swiss Prot
Q95LI5-1
别名
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
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