Biotinylated Recombinant Human ACE-2 Protein (RP02269)

Biotinylated Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinlylated human ACE2 is greater than 95% as determined by SEC-HPLC.

Immobilized SARS-COV-2 S1, His Tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human ACE2, His Tag with EC₅₀ of 0.24μg/ml determined by ELISA.

Immobilized SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC₅₀ of 0.06μg/ml determined by ELISA.

All(4)|

货号: RP02269

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折叠内容

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详细信息

种属

Human

表达宿主

HEK293 cells

描述

Biotinylated Recombinant Human ACE2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln18-Ser740) of Human ACE2 fused with a His tag and Avi at theC-terminal.

标签

C-His&Avi

纯度

> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC

内毒素

< 1 EU/μg of the protein by LAL method.

生物活性

Immobilized n-COV RBD at 1 μg/mL (100 μL/Well). Dose response curve for Biotinylated ACE2 with the EC₅₀ of 0.1 μg/mL determined by ELISA.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

储存

Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

基因ID

59272

Swiss Prot

Q9BYF1

别名

ACE2; ACEH; angiotensin-converting enzyme 2;ACEH

研究领域

Biotinylated Recombinant Human ACE-2 Protein (RP02269)

产品导航

Biotinylated Recombinant Human ACE-2 Protein (RP02269)

文献引用 (1)

详细信息

蛋白复溶计算器

背景信息

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