Recombinant Cynomolgus CD3 epsilon/CD3E Protein (RP02318)

说明书收藏

Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC₅₀ of 39.4ng/ml determined by ELISA.

All(3)|

货号: RP02318

详细信息

种属

Cynomolgus

表达宿主

HEK293 cells

Calculated MW

36.9 kDa

Observed MW

43-48 kDa

标签

C-hFc

纯度

> 95% by Tris-Bis PAGE

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Cynomolgus CD3 Epsilon Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln22-Asp117) of Cynomolgus CD3 Epsilon fused with a hFc tag at the C-terminal.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Immobilized Cyno CD3 Epsilon,hFc Tag at 1 μg/mL (100 μL/Well). Dose response curve for Biotinylated Anti-CD3E Ab. with the EC₅₀ of 0.2 μg/mL determined by ELISA.

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。