Recombinant Human NKG2A&KLRD1/CD159a&CD94 Protein (RP02451)

说明书收藏

Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.

Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).

Immobilized Human NKG2A&CD94, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC₅₀ of 12.4ng/ml determined by ELISA.

Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 80.0ng/ml.

All(5)|

货号: RP02451

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

56.9 kDa

Observed MW

70-90 kDa

标签

C-mFc

纯度

> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human NKG2A&KLRD1/CD159a&CD94 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser34-Ile179)&(Arg100-Leu233) of Human NKG2A&KLRD1/CD159a&CD94 (Accession #) fused with mFc tag at the C-terminal.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Immobilized Human NKG2A&CD94,His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-NKG2A&CD94 Ab with the EC₅₀ of 0.2 μg/mL determined by ELISA.

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

基因ID

3821&3824

Swiss Prot

P26715&Q13241

别名

CD159 antigen-like family member A; CD159a antigen; CD159a;NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor;CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D; member 1; KLRD1; KP43; NK cell receptor