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Recombinant Human IL-37/IL-1F7 Protein (RP02509)

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Recombinant Human IL-37/IL-1F7 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 20 kDa.

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货号: RP02509
促销价：   ￥1600
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详细信息

种属
Human
表达宿主
E. coli
描述
Recombinant Human IL-37 Protein is produced by Escherichia coli expression system. The target protein is expressed with sequence of Human IL-37 (Accession #NP_775297.1) fused with His at C terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
<0.1EU/μg
生物活性
Measured by its ability to inhibit IL-8 secretion in human PBMCs. The ED50 for this effect is <0.9 μg/mL.
制剂
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Decreasing synthesis of IL-37 in PBMCs will cause increasing production of pro-inflammatory cytokines such as IL-1 alpha, IL-1 beta, IL-6 and TNF- alpha. The IL-37 work as an innate inflammatory inhibitor is also proved by its high expression in synovial tissue from rheumatoid arthritis patients. Full length IL-37 locates mainly in the cytoplasm, and it can translocate to the nucleus after activation through cleavage by CASP1, then exerts its activity by directly interacting with SMAD3.

基因ID
27178
Swiss Prot
Q9NZH6
别名
IL37;FIL1;FIL1(ZETA);FIL1Z;IL-1F7;IL-1H;IL-1H4;IL-1RP1;IL-37;IL1F7;IL1H4;IL1RP1
研究领域
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