Biotinylated Recombinant Human Siglec-10 (R119A) Protein (RP02585B)

说明书收藏

Biotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

Immobilized Anti-Siglec-10 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC₅₀ of 61.7ng/ml determined by ELISA.

All(3)|

货号: RP02585B

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

62.64 kDa

Observed MW

68-80 kDa

标签

C-His&Avi

纯度

> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20 mM Tirs 150 mM NaCl, 200 mM L-arginine, pH 8.0.

描述

Biotinylated Recombinant Human Siglec-10 (R119A) Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met17-Thr546) of Human Siglec-10 (R119A) fused with a His and Avi tag at the C-terminal.

储存

Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

基因ID

89790

Swiss Prot

Q96LC7-1

别名

SLG2; SIGLEC10; MGC126774; PRO940