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Recombinant Cynomolgus/Rhesus macaque TNFRSF7/CD27 Protein (RP02610)

Cynomolgus/Rhesus macaque CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus/Rhesus macaque CD27 is greater than 95% as determined by SEC-HPLC.

Immobilized Cynomolgus CD27 Ligand, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.5ng/ml determined by ELISA.

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货号: RP02610
促销价:   ¥3200
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详细信息

种属
Cynomolgus/Rhesus macaque
表达宿主
HEK293 cells
Calculated MW
46.5 kDa
Observed MW
60-70 kDa
标签
C-hFc
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
描述
Recombinant Cynomolgus/Rhesus macaque CD27/TNFRSF7 Protein is expressed from Expi293 with hFc tag at the C-terminal.;It contains Ala20-Arg191.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co?stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Swiss Prot
别名
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
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