Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein (RP02641)

说明书收藏

Cynomolgus Fc gamma RIIA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus Fc gamma RIIA is greater than 95% as determined by SEC-HPLC.

Rituximab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIIA, His Tag with an affinity constant of 3.65 μM as determined in SPR assay (Biacore T200).

All(3)|

货号: RP02641

详细信息

种属

Cynomolgus

表达宿主

HEK293 cells

Calculated MW

21.3 kDa

Observed MW

30-40 kDa

标签

C-His

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein is expressed from Expi293 with His tag at the C-terminal.；It contains Gln28-Pro208.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

基因ID

102143568

Swiss Prot

Q8SPW4-1

别名

CD32MGC23887; CDw32fc-gamma-RIIa; Fc gamma RIIA; FCG2; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; FCG2; CD32A