Recombinant Cynomolgus LILRB4/ILT-3/CD85k Protein (RP02649)

说明书收藏

Cynomolgus LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus LILRB4 is greater than 95% as determined by SEC-HPLC.

Immobilized Cynomolgus LILRB4, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC₅₀ of 0.38μg/ml determined by ELISA.

All(3)|

货号: RP02649

详细信息

种属

Cynomolgus

表达宿主

HEK293 cells

Calculated MW

27.2 kDa

Observed MW

35-42 kDa

标签

C-His

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Cynomolgus LILRB4/ILT-3/CD85k Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln22-Glu259) of Cynomolgus LILRB4 fused with His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

LILRB4,also known as CD85k and LIR-5, ILT3,? is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

基因ID

107128256

Swiss Prot

XP_015297198.1

别名

HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K