Recombinant Cynomolgus IL-23A&Mouse IL-12B Protein (RP02725)

说明书收藏

Cynomolgus IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.

Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).

All(3)|

货号: RP02725

详细信息

种属

Cynomolgus&Mouse

表达宿主

HEK293 cells

Calculated MW

19.5 kDa(Cynomolgus IL-23 alpha), 35.8 kDa(Mouse IL-12 beta)

Observed MW

22 kDa(Cynomolgus IL23 alpha), 40-50 kDa(Mouse IL12 beta)

标签

N-His

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Cynomolgus IL-23A&Mouse IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val22-Pro189(Cynomolgus IL-23A)&Met23-Ser335(Mouse IL-12B)) of cynomolgus IL-23A&mouse IL-12B (Accession #A0A2K5TLV4-1(Cynomolgus IL-23A)&P43432-1(Mouse IL-12B)) fused with His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

基因ID

16160&102128555

Swiss Prot

A0A2K5TLV4-1(Cynomolgus IL-23 alpha)&P43432-1(Mouse IL-12 beta)

别名

IL23 alpha; IL12 beta; IL23 alpha&IL12 beta