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Recombinant Cynomolgus FAP Protein (RP02730)

Cynomolgus FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus FAP is greater than 95% as determined by SEC-HPLC.

Immobilized Cynomolgus FAP at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 31.2ng/ml determined by ELISA.

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货号: RP02730
促销价:   ¥5250
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详细信息

种属
Cynomolgus
表达宿主
HEK293 cells
Calculated MW
86.2 kDa
Observed MW
80-100 kDa
标签
N-His
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
描述
Recombinant Cynomolgus FAP Protein is expressed from Expi293 with His tag at the N-terminal.;It contains Leu26-Asp760.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

基因ID
Swiss Prot
别名
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA