Recombinant Human Alkaline phosphatase (Intestinal type)/ALPI Protein (RP02744)

说明书收藏

Human Alkaline phosphatase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Alkaline phosphatase is greater than 95% as determined by SEC-HPLC.

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline Phosphatase, His Tag with an affinity constant of 0.96 μM as determined in SPR assay (Biacore T200).

All(3)|

货号: RP02744

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

54.5 kDa

Observed MW

63-73 kDa

标签

N-His&Flag

纯度

> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC

内毒素

Less than 1EU per μg by the LAL method.

描述

Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Val20-Asp503) of Human Alkaline phosphatase (Intestinal type) /ALPI (Accession # P09923) fused with His tag and Flag tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

基因ID

248

Swiss Prot

P09923

别名

ALPI，Intestinal-type alkaline phosphatase; IAP; Intestinal alkaline phosphatase;