Recombinant Human RNF43 Protein (RP02777)

Human RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.

Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC₅₀ of 0.4μg/ml determined by ELISA.

Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC₅₀ of 16.1ng/ml determined by ELISA.

All(4)|

货号: RP02777

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

45.8 kDa

Observed MW

55-60 kDa

标签

C-hFC

纯度

> 95% as determined by HPLC

内毒素

<1EU/μg

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human RNF43 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Tyr197) of human RNF43 (Accession #NP_001292473.1) fused with a hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Immobilized Human R spondin 1, His Tag at 5 μg/mL (100μL/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC₅₀ of 0.4 μg/mL determined by ELISA.
2.Immobilized Human R-Spondin 3, His Tag at 1 μg/mL (100μL/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC₅₀ of 16.1 ng/mL determined by ELISA.

复溶

Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.

基因ID

54894

Swiss Prot

Q68DV7

别名

URCC; SSPCS; RNF124

研究领域