产品中心 / 产品类型 / 重组蛋白 / 泛素化相关试剂

活性验证

Recombinant Human RNF43 Protein (RP02777)

说明书 联系我们 收藏

Human RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.

Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4μg/ml determined by ELISA.

Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA.

All(4)|
货号: RP02777
促销价：   ￥2700
货    期：在线咨询货期
抗体定制服务咨询 |
商城订购

详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human RNF43 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Tyr197) of human RNF43 (Accession #NP_001292473.1) fused with a hFc tag at the C-terminus.
标签
C-hFC
纯度
> 95% as determined by HPLC
内毒素
<1EU/μg
生物活性
1.Immobilized Human R spondin 1, His Tag at 5 μg/mL (100μL/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4 μg/mL determined by ELISA.
2.Immobilized Human R-Spondin 3, His Tag at 1 μg/mL (100μL/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1 ng/mL determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
=
÷

背景信息

RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.

基因ID
54894
Swiss Prot
Q68DV7
别名
URCC; SSPCS; RNF124
研究领域

    最新浏览的产品

    >