Recombinant Human Aldehyde reductase/AKR1B1 Protein (RP02938)

说明书联系我们收藏

Recombinant Human Aldehyde reductase/AKR1B1 Protein was determined by SDS-PAGE with Coomassie Blue,

All(1)|

货号: RP02938

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

37.9 kDa

Observed MW

36 kDa

标签

N-His

纯度

> 90 % as determined by SDS-PAGE

内毒素

Please contact us for more information.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, 20% glycerol, pH 7.5.

描述

Recombinant Human Aldehyde reductase/AKR1B1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Phe316) of human Aldehyde reductase/AKR1B1 (Accession #NP_001619.1) fused with 6×His tag at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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背景信息

Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.

基因ID

231

Swiss Prot

P15121

别名

AKR1B1; ADR; ALDR1; ALR2; AR; aldose reductase;ADR;ALDR1;ALR2;AR