Recombinant Human KRAS-WT(GDP load) Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 23.5 kDa.
KRAS-WT activity test using HTRF method. The KRAS-WT activity was assayed with HTRF technology. The reaction was performed by incubating the KRAS- WT protein, GTP, cRAF and beads at 25℃ for 60 min, then reading Ratio 665/620nm signal with BMG.
KRAS-WT activity test using HTRF method. The KRAS-WT activity was assayed with HTRF technology. The reaction was performed by incubating the KRAS- WT protein, GTP, cRAF and beads at 25℃ for 60 min, then reading Ratio 665/620nm signal with BMG.
