纯度
≥ 95 % as determined by SDS-PAGE.
内毒素
Please contact us for more information.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Tris, 150mM NaCl, 1mM DTT, 10% glycerol, pH7.4
描述
Recombinant Human GTPase KRas/KRAS (G12R) Protein is produced by E. coli expression system. The target protein is expressed with sequence of Human GTPase KRas/KRAS (G12R) (Accession #)fused with N-terminal 8x his tag+ TEV cleavage site and C-terminal Avi tag.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
（1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
KRAS-G12R activity test using HTRF method.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
