表达宿主
Baculovirus-Insect Cells
Calculated MW
38 kDa(PRkAG1), 30 kDa(PRkAB1), 92 kDa(PRkAA1)
Observed MW
38 kDa(PRkAB1), 40 kDa(PRkAG1), 95 kDa(PRkAA1)
纯度
≥ 85 % as determined by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Supplied as sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH 7.5.
描述
Recombinant Human AMPK (G1/B1/A1) Heterotrimer Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Pro331&Met1-Ile270&Met1-Gln559) of human AMPK (Accession #) fused with GST and His tag at the N-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
（1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。
生物活性
The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
