Recombinant Human Gelsolin/GSN Protein (RP03183)

说明书联系我们收藏

Recombinant Human Gelsolin/GSN Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 115-125 KDa.

All(1)|

货号: RP03183

详细信息

种属

Human

表达宿主

HEK293 cells

描述

Recombinant Human Gelsolin/GSN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala28-Ala782) of Human Gelsolin/GSN (Accession #NP_000168.1) fused with hFc tag at the C-terminus.

标签

C-mFc

纯度

> 90% by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, 5% Trehalose, 0.1% Triton X-100, pH 8.0. Contact us for customized product form or formulation.

储存

Store the lyophilized protein at -20°C to -80 °C for 12 months.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

Gelsolin is a calcium-activated actin filament severing and capping protein found in many cell types and as a secreted form in the plasma of vertebrates. Gelsolin is composed of six of these domains, termed G1-6. Some reaseraches show that gelsolin can act as a transcriptional cofactor in signal transduction and its own expression and function can be influenced by epigenetic changes. The difference in the expression levels of cytoplasmic and plasma gelsolin suggests that these two different forms of gelsolin may play different roles after cardiac injury. Plasma gelsolin, as an important component of the EASS, may have evolved to rapidly clear actin filaments from the circulation that are released by injured or dead cells.

基因ID

2934

Swiss Prot

P06396-1

别名

Gelsolin; GSN; AGEL; Actin-depolymerizing factor; ADF; Brevin

研究领域

Recombinant Human Gelsolin/GSN Protein (RP03183)

产品导航

Recombinant Human Gelsolin/GSN Protein (RP03183)

详细信息

蛋白复溶计算器

背景信息

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