Recombinant Human R-spondin-2/RSPO2 Protein (RP03266)

Recombinant Human R-spondin-2/RSPO2 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions.

All(1)|

货号: RP03266

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

47.66 kDa

Observed MW

55-60 kDa

标签

C-hFC

纯度

> 90% by SDS-PAGE.

内毒素

< 0.01 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

描述

Recombinant Human R-spondin-2/RSPO2 Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ala32-Gly205) of human R-spondin-2 (Accession #Q6UXX9) fused with hFc tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED₅₀ is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a Surrogate.

复溶

Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

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折叠内容

背景信息

R-spondins are secretory proteins localized in the endoplasmic reticulum and Golgi bodies and are processed through the secretory pathway. The RSPO family comprises four proteins, i.e., R-spondin 1-4, which are important secreted regulators of the Wnt/尾-catenin and Wnt/PCP signaling pathway and govern various functions and are involved in vital cellular processes such as stem cell renewal, morphogenesis and, tumorigenesis. Among the R-spondin family, R-spondin-2, is a key protein that regulates ligand-dependent 尾-catenin signaling. And it has been reported to play a pivotal role in myogenesis, osteoblast differentiation, and limb specification during embryonic development.

基因ID

340419

Swiss Prot

Q6UXX9-1

别名

R-spondin-2; Roof plate-specific spondin-2; hRspo2;RSPO2; UNQ9384/PRO34209

研究领域