Recombinant Human Sortilin/SORT1 Protein (RP03274)

Recombinant Human Sortilin/SORT1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 85-95 KDa.

Immobilized Recombinant Human Sortilin/SORT1 Protein at 2 μg/mL (100 μL/well) can bind biotinylated Recombinant Human Progranulin Protein, the EC₅₀ is 500-1500 ng/mL.

All(2)|

货号: RP03274

详细信息

种属

Human

表达宿主

HEK293 cells

描述

Recombinant Human Sortilin/SORT1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser78-Asn755) of human Sortilin/SORT1 (Accession #Q99523-1) fused with His tag at the C-terminus.

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

生物活性

Immobilized Recombinant Human Sortilin/SORT1 Protein at 2 μg/mL (100 μL/well) can bind biotinylated Recombinant Human Progranulin Protein, the EC₅₀ is 500-1500 ng/mL.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

The Sortilin is a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer.

基因ID

6272

Swiss Prot

Q99523-1

别名

Sortilin; SORT1; Glycoprotein 95; Gp95; Neurotensin receptor 3; NT3; NTR3

研究领域

Recombinant Human Sortilin/SORT1 Protein (RP03274)

产品导航

Recombinant Human Sortilin/SORT1 Protein (RP03274)

详细信息

蛋白复溶计算器

背景信息

SORT1 相关产品