Recombinant Monkeypox virus M1R Protein (RP03292)

说明书联系我们收藏

Recombinant Monkeypox virus M1R Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-40 kDa.

The purity of Monkeypox virus M1R is greater than 95% as determined by SEC-HPLC.

All(2)|

货号: RP03292

详细信息

种属

Monkeypox virus

表达宿主

HEK293 cells

Calculated MW

20.41 kDa

Observed MW

20-40 kDa

标签

C-His

纯度

> 95% by Tris-Bis PAGE and HPLC.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.

描述

Recombinant Monkeypox virus M1R Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly2-Gly183) of Monkeypox virus M1R (Accession #QJQ40223.1) fused with His tag at the C-terminus.

储存

Store at -20°C. Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. M1R is homologous to the vaccinia virus L1 protein, a transmembrane protein found on the surface of mature IMV particles. And M1R is the component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation.

基因ID

928968

Swiss Prot

Q8V502

别名

M1R; Monkeypox virus M1R; MPXV M1R