Recombinant Human PIK3C3 Kinase was resolved with SDS-PAGE under reducing (Lane 1) and non-reducing (Lane 2) conditions.
The activity of PK3C3 is based on the ADP-GLO kinase activity assay quantifies kinase activity by measuring the conversion of ATP to ADP catalyzed by the kinase. Specific reagents are used to convert the ADP in the reaction back to ATP, resulting in the production of a luminescent signal.
Phosphatidylinositol 3-kinase catalytic subunit type 3 is an enzyme subunit that in humans is encoded by the PIK3C3 gene. It's a class III phosphoinositide 3-kinase. PI3Ks include class I PI3Ks (PIK3C1), class II PI3Ks (PIK3C2), and PIK3C3. PIK3C1 inhibits autophagy through phosphorylation of the 3-position of the inositol headgroup in phosphatidylinositol (PtdIns) 4,5-bisphosphate in the regulation of the AKT-mTOR pathway. However, PIK3C2 and PIK3C3 mediate the initiation of autophagy by phosphorylating PtdIns to PI3P. PIK3C3-C1 or PIK3C3-C2 is formed by the combination of different regulatory subunits. PIK3C3-C1, which is comprised of the PIK3C3 catalytic subunit and regulator subunits of PIK3R4, BECN1, Atg14, NRBF2, and AMBRA1, is essential for autophagy initiation. The major role of PIK3C3-C2 is to regulate vesicle trafficking and autophagosome formation.
