Recombinant Human RNASE1 Protease (RP03548LQ)

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货号: RP03548LQ

详细信息

种属

Human

表达宿主

Mammalian

Calculated MW

15.6 KDa

Observed MW

17-33 KDa

标签

C-His

纯度

≥ 95 % as determined by SDS-PAGE.

内毒素

< 1 EU/μg of the protein by LAL method.

制剂

Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.

描述

Recombinant Human RNASE1 Protease is produced by Mammalian expression system. The target protein is expressed with sequence (Lys29-Thr156) of Human RNASE1 (Accession #P07998) fused with His tag at the C-Terminus.

储存

Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. （1）未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周；
（2）干粉蛋白复溶浓度建议在0.1-0.5 mg/mL，不能低于0.1mg/mL，长期储存请添加保护剂（如0.1%BSA），并分装保存，避免反复冻融；
（3）液体蛋白的保存温度及效期请参考说明书，如对液体蛋白浓度有要求，请提前咨询销售或者技术支持。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA.

基因ID

6035

Swiss Prot

P07998

别名

RNASE1; RIB1; RNS1，Ribonuclease pancreatic，RNase 1; Ribonuclease A; RNase A