≥ 95 % as determined by Reducing and No-Reducing SDS-PAGE.
内毒素
< 0.01 EU/μg of the protein by LAL method.
制剂
Supplied as 50 mM HEPES (pH 7.5), 500 mM NaCl, 20% glycerol,1 mM
DTT.
描述
Recombinant Human Transketolase/TKT Protein is produced by E. coli Cells system. The target protein is expressed with sequence (Glu2-ALa623) of Human Transketolase/TKT (Accession #P29401) fused His tag at the N-Terminus.
储存
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
(1)未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周;
(2)干粉蛋白复溶浓度建议在0.1-0.5 mg/mL,不能低于0.1mg/mL,长期储存请添加保护剂(如0.1%BSA),并分装保存,避免反复冻融;
(3)液体蛋白的保存温度及效期请参考说明书,如对液体蛋白浓度有要求,请提前咨询销售或者技术支持。
蛋白复溶计算器
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
背景信息
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.