Recombinant human NEMO (L329P)/IKBKG protein was determined by SDS-PAGE with Coomassie Blue.
NF-κB essential modulator (NEMO) is a regulatory subunit within IκB kinase (IKK). IKK is also composed of two unrelated catalytic subunits called IKKα and IKKβ. NEMO is additionally referred to as IKKγ, IKKAP1, or FIP-3. IKK is responsible for activating the transcription factor, NF-κB, which is involved in immune response. NEMO prefers to bind K63-linked or linear polyubiquitin chains. The L329P substitution abolishes NEMO interacts with linear polyUb chains and long K63 polyubiquitin chains.
