Polyubiquitinated UbE2S (Ubn (K11-linked)-UbE2S) is a polyubiquitinated substrate in which various K11-linked polyUb chains were conjugated on the Ub conjugating enzyme UbE2S. It can be used as a substrate to monitor deubiquitination mediated by deubiquitinating enzymes, or to determine binding of K11-linked polyUb chains by proteins that have Ub interacting domains.