The differentiation medium containing RSPO1 (500 ng/mL), Noggin (100 ng/mL), and EGF (100 ng/mL) was used to induce the differentiation of mid-hindgut cells into small intestinal organoids. After 7 days of induction, the expression of markers CDX2, MUC2, CgA, and E-Cad was assessed by immunofluorescence.
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