Recombinant Salt Active SuperNuclease (RPT0032LQ)

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Recombinant Salt Active SuperNuclease was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-35 kDa.

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货号: RPT0032LQ

详细信息

种属

Halophilic microorganisms

表达宿主

E.coli

Calculated MW

25.4 kDa

Observed MW

25-35 kDa

标签

纯度

≥ 95% by SDS-PAGE.

内毒素

≤ 10 EU/mg.

制剂

Supplied as 50 mM Tris, 500 mM NaCl, 5 mM MgCl₂, 50% (v/v) Glycerol, pH 7.4.

描述

Recombinant Salt Active SuperNuclease is produced by E.coli expression system. The target protein is expressed with sequence of Halophilic microorganisms.

储存

Store at -20°C. This product is stable at -20°C up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

One unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37℃, using overdose DNA substrate in a buffer with 50 mM Tris-HCl (pH 8.0, 25℃), 5 mM MgCl₂, and 500 mM NaCl. The enzyme activity is ≥ 3×10⁵ U/mg.

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

背景信息

Recombinant Salt Active SuperNuclease is a nonspecific endonuclease from a halophilic microorganisms, which is produced in E. coli. Because of the high affinity with cation exchange resins, this enzyme can be removed easily from biological samples such as adeno-associated virus (AAV) and lentivirus. Salt Active SuperNuclease has optimal activity at salt concentrations between 350~650 mM.

别名

Salt Active SuperNuclease; SASN; High Salt; Endonuclease; Nuclease; 高盐全能核酸酶