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ATF4 Polyclonal Antibody

概述
货号: A0201
宿主: Rabbit
同种型: IgG
ABclonal:Western blot - ATF4 Polyclonal Antibody (A0201)
ABclonal:Immunofluorescence - ATF4 Polyclonal Antibody (A0201)
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背景

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.

免疫原信息

免疫原Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).
序列MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP
基因ID468
Swiss ProtP18848
别名ATF4; CREB-2; CREB2; TAXREB67; TXREB; activating transcription factor 4

应用

反应物种Human, Mouse, Rat
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Mouse , Rat)

推荐稀释比
WB 1:500 - 1:2000
IF 1:50 - 1:200
理论分子量38kDa
实际分子量50kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
实验方法Western blotting    
阳性样本HeLa
细胞定位Cell membrane,Cytoplasm,Nucleus,centrosome,cytoskeleton,microtubule organizing center
纯化方式Affinity purification

ATF4 Polyclonal Antibody 检测图片

ABclonal:Western blot - ATF4 Polyclonal Antibody (A0201) }

Western blot - ATF4 Polyclonal Antibody (A0201)

Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunofluorescence - ATF4 Polyclonal Antibody (A0201) }

Immunofluorescence - ATF4 Polyclonal Antibody (A0201)

Immunofluorescence analysis of HeLa cells using ATF4 antibody (A0201) at dilution of 1:100. Blue: DAPI for nuclear staining.

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