CD8+ T cells were initially isolated from fresh C57BL/6 mouse splenocytes using anti-CD8 rabbit monoclonal antibody (A23904). CD8+ T cells were activated for 2 days by anti-CD3 and CD28 antibodies, Activated CD8+ T cells demonstrated elevated expression of CD25 (left panel) and CD69 (right panel) using flowcytometry. ABflo® 594-conjugated anti-CD25 rabbit monoclonal antibody (A23809) and ABflo® 488-conjugated anti-CD69 rabbit monoclonal antibody (A24938) were used in flowcytometry assays.
Enables interleukin-2 binding activity and interleukin-2 receptor activity. Acts upstream of or within several processes, including Notch signaling pathway; activation-induced cell death of T cells; and regulation of T cell proliferation. Located in external side of plasma membrane. Is expressed in bone marrow. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in immunodeficiency 41; lymphopenia; multiple sclerosis; type 1 diabetes mellitus; and type 1 diabetes mellitus 10. Orthologous to human IL2RA (interleukin 2 receptor subunit alpha).
i_FC_08.jpg?t=1790725009)
