概述
货号: AC004
宿主: Mouse
同种型: IgG
ABclonal:Western blot - ACTB Monoclonal Antibody (AC004)
ABclonal:Western blot - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC004)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC004)
优惠价
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背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

免疫原信息

免疫原Fusion protein of human β-actin
序列Email for sequence
基因ID60
Swiss ProtP60709
别名ACTB; BRWS1; PS1TP5BP1; beta actin

应用

反应物种Human, Mouse, Rat
验证应用WBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
用户验证的应用

WB (Human , unknown , Mouse , Tongue sole , Murine , Pacific oyster , Rat , Human , Citrus sinensis , Pig , grass carp)

IF (Human , Mouse)

IP (Human)

IF (Human)

IHC (Human)

推荐稀释比
WB 1:10000 - 1:100000
IHC 1:100- 1:200
IF 1:50- 1:200
抗体稀释方法Put 5ul AC004 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot
理论分子量41kDa
实际分子量42kDa
存储缓冲液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
实验方法Western blotting    
阳性样本HeLa,TE-1,293T,NIH/3T3,Mouse brain,Mouse testis,C6,Rat brain,Rat lung
细胞定位Cytoplasm,cytoskeleton
纯化方式Affinity purification

ACTB Monoclonal Antibody 检测图片

ABclonal:Western blot - ACTB Monoclonal Antibody (AC004) }

Western blot - ACTB Monoclonal Antibody (AC004)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC004) at 1:80000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - ACTB Monoclonal Antibody (AC004) }

Western blot - ACTB Monoclonal Antibody (AC004)

Western blot analysis of extracts of NIH/3T3 cells, using β-actin antibody (AC004) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat heart using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded human stomach using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse heart using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat liver using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat testis using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004) }

Immunofluorescence - ACTB Monoclonal Antibody (AC004)

Immunofluorescence analysis of C6 cells using ACTB Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004) }

Immunofluorescence - ACTB Monoclonal Antibody (AC004)

Immunofluorescence analysis of HeLa cells using ACTB Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004) }

Immunofluorescence - ACTB Monoclonal Antibody (AC004)

Immunofluorescence analysis of L929 cells using ACTB Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

评论列表

CustomerJul 052016
Review for ACTB Monoclonal Antibody(AC004)5
Cat.No
AC004
Application
Immunohistochemistry(IHC)
Sample
Mouse kidney
Dilution
1:200
Incubatition time
12h
Temperature
unknow
Exposure
unknow
Observed Band
unknow kDa
Remarks
Immunohistochemistry analysis of Mouse kidney, using β-actin Monoclonal Antibody.

More

CustomerJul 042016
Review for ACTB Monoclonal Antibody(AC004)5
Cat.No
AC004
Application
Immunohistochemistry(IHC)
Sample
Rat kidney
Dilution
1:300
Incubatition time
12h
Temperature
unknow
Exposure
unknow
Observed Band
unknow kDa
Remarks
Immunohistochemistry analysis of Rat kidney, using β-actin Monoclonal Antibody.

More

>

Reviews List

CustomerJul 052016
Review for ACTB Monoclonal Antibody(AC004)5
Cat.No
AC004
Application
Immunohistochemistry(IHC)
Sample
Mouse kidney
Dilution
1:200
Incubatition time
12h
Temperature
unknow
Exposure
unknow
Observed Band
unknow kDa
Remarks
Immunohistochemistry analysis of Mouse kidney, using β-actin Monoclonal Antibody.

More

CustomerJul 042016
Review for ACTB Monoclonal Antibody(AC004)5
Cat.No
AC004
Application
Immunohistochemistry(IHC)
Sample
Rat kidney
Dilution
1:300
Incubatition time
12h
Temperature
unknow
Exposure
unknow
Observed Band
unknow kDa
Remarks
Immunohistochemistry analysis of Rat kidney, using β-actin Monoclonal Antibody.

More

>

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